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Cytogenetics, Chromosome Analysis, Bone Marrow
Laboratory:
Cytogenetics
Soft Order Code:
CGNBM
Synonyms:
Cytogenetics, Bone Marrow Karyotype, Bone Marrow
Chromosome Analysis, Bone Marrow for Malignancy Chromosome Analysis for Hematologic Disorders, Bone Marrow
Container:
Chromosome Media, heparinized syringe, or green top (sodium heparin) tube
Normal Volume:
3 - 5 mL bone marrow
Minimum Volume:
1 mL bone marrow
Offsite Collection:
MLabs client and off site UM Health Center requests for this test will be sent to Mayo Medical Laboratories unless alternate arrangements have been made. Specimen transport should be arranged so that the specimen is received by MLabs the same day it is collected. Call for a STAT courier if necessary. Collect bone marrow using aseptic technique. Place 1 - 5 mL bone marrow aspirate in a green top (sodium heparin) tube. Invert the tube several times to prevent clotting. Send at room temperature as soon as possible. Do not allow specimen to overheat or freeze. Clotted samples, samples containing <5 million dividing cells, and samples that are greater than 24 hours old are suboptimal. Please complete an MLabs Cytogenetics Requisition and Clinical History Form available online at http://mlabs.umich.edu/files/pdfs/REQ-mlabs_cytogen_req_refr.pdf and submit it with the specimen.
Onsite Collection (UMHS Hospitals Only):
Chromosome Media tube. Sterile syringe rinsed with sodium heparin or green top vacutainer with sodium heparin. For outpatient testing, complete a MiChart test request; for inpatient testing complete a Cytogenetics Laboratory Requisition available online at http://www.pathology.med.umich.edu/handbook/Tables/UM_REQ_CYTOGENETICS.pdf and submit it with the specimen; pertinent medical findings and diagnosis must be included. All pediatric bone marrow specimens must be transported in Chromosome Media available from Specimen Processing.
Days Set Up:
Monday - Friday, 8:00am - 4:00pm. If necessary a STAT courier may be arranged for processing a sample on a priority basis. Call the laboratory at 763-5805 for additional information.
Analytic Time:
3 weeks
Reference Range:
*Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Interpretive report provided.
Test Usage:
Identification of specific chromosomal abnormalities associated with hematologic disease. Bone Marrow specimens are most commonly sent as part of the evaluation of acute leukemia (lymphoid or myeloid), MDS, or myeloproliferative disorders including chronic myelogenous leukemia, in the latter case to look for a Philadelphia chromosome.
Test Limitations:
Cells must be capable of dividing.
Test Methodology:
Unstimulated short term cell culture and chromosome analysis. Fluorescence In Situ Hybridization (FISH) when indicated.
Additional Information:
Appropriate FISH testing will be performed at an additional charge when indicated. FISH testing is available for a number of oncology probes, including BCR/ABL, PML/RARA, TEL/AML1, and MLL (see Cytogenetics, FISH for Malignancy). For additional information on FISH testing and available probes, contact the Cytogenetics Laboratory. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. MLabs client and off site UM Health Center requests for this test may be sent to Mayo Medical Laboratories BM (8506).
CPT Code:
88262 Chromosome Analysis, 88237 Tissue Culture
Inpatient Fee Code:
36526, 36542
Outpatient Fee Code:
36526, 36542
MLabs Fee Code:
36526 Chromosome Analysis, 36542 Tissue Culture
Rejection Criteria:
Clotted or hemolyzed specimen, specimen over 24 hours old, or those from septic or transfused patients.
Database ID:
278

For Technical Questions or Comments Directly Dealing With The Handbook E-mail: jsica@umich.edu