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Extractable Nuclear Antibody Panels
Laboratory:
Immunopathology
 Supporting Articles
Order Code:
ENA5/ENA7/ENA10
Synonyms:
SmRNP Antibody U1RNP Antibody
Centromere B Antibody Anti-Centromere
Scl-70 Antibody Anti-Scl 70
Sjogrens syndrome-A extractable nuclear Ab Jo-1 Antibody
Chromatin Antibody Ribosomal Protein Antibody
Anti-SSB (La) Anti-SSA (Ro)
Ro Antibody La Antibody
RNP Antibody Anti-Ro (SSA)
Anti-RNP (Ribonucleoprotein) Anti-Sm (Smith)
Sm (Smith) Antibody Anti-La (SSB)
SS-A Antibody SS-B Antibody
Anti-SmRNP Anti-Jo 1
Scleroderma Antibody Anti-Synthetase Syndrome
Anti-HisRS (Jo-1) Anti-tRNA Synthetase
Sjogrens syndrome-B extractable nuclear Ab Ribonucleoprotein extractable nuclear Ab
Smith extractable nuclear Ab
Container:
SST tube
Normal Volume:
0.5 mL serum
Minimum Volume:
0.25 mL serum
Offsite Collection
Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Please indicate panel or specific antibody testing to be performed.
Onsite Collection (UMHS Hospitals Only)
Collect specimen in SST tube. Please indicate panel or specific antibody testing to be performed.
Days Set Up:
Monday - Friday
Analytic Time:
1 day
Reference Range:
Negative
Test Usage:
Useful diagnostic and prognostic information can be gained in ANA positive patients by knowing the specificity of the autoantibodies that are detected. Ideally, collagen vascular disease diagnosis is based on a combination of clinical symptoms and laboratory testing. It is not cost effective to follow up every positive ANA with assays for all 10 possible detectable antibody specificities. MLabs promotes a more cost effective strategy an ENA 5 Antibody Panel (includes SS-A, SS-B, Sm, RNP, and SmRNP) (order code ENA5). These antibodies will account for the vast majority of significant positive results. Other ENA antigens such as Scl-70, Centromere B, Jo-1, and Ribosomal P should not be routinely screened for, but ordered as individual tests as the differential diagnosis dictates. The laboratory recommends either anti-dsDNA (order code ADNA) or crithidia DNAIFA (order code DNAIF) to detect and quantify anti-dsDNA.
Test Methodology:
Multiplex Flow Immunoassay
Additional Information:
See Extractable Nuclear Antibody Testing.available online at http://www.pathology.med.umich.edu/handbook/Tables/ANA-ENA-08.pdf. ENA5 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, and RNP. ENA7 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, and Scleroderma (Scl-70). ENA10 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, Scleroderma (Scl-70), Centromere B, Chromatin, and Ribosomal P. The following antibodies may also be ordered individually: Centromere B (CENT B), Jo-1 (JO-1), Ribosomal P (RIBO P), RNP (RNP), Scleroderma (SCL-70), Smith (SMITH), SS-A (SS-A), and SS-B (SS-B).
LOINC:
Z32-3 ENA5, Z1002-5 ENA7, 8076-2 JO-1, 8092-9 SCL-70, 16137-2 CENT B, Z1001-7 CHROM, 5130-0 DSDNA, 31592-9 RIBO P
CPT Code
86235 EACH ANTIBODY
Inpatient Fee Code
20572 ENA5, 20573 ENA7, KA002 ENA10, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
Outpatient Fee Code
20572 ENA5, 20573 ENA7, KA002 ENA10, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
MLabs Fee Code
20572 ENA5, 20573 ENA7, KA002 ENA10, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
Database ID:
402

For Technical Questions or Comments Directly Dealing With The Handbook E-mail: jsica@umich.edu