|
Laboratory:
Immunopathology
|
 Supporting Articles |
ENA5/ENA7/ENA11
| SmRNP Antibody | ds-DNA |
| U1RNP Antibody | Anti-ds-DNA |
| Centromere B Antibody | Anti-Centromere |
| Scl-70 Antibody | Anti-Scl 70 |
| Sjogrens syndrome-A extractable nuclear Ab | Jo-1 Antibody |
| Chromatin Antibody | Ribosomal Protein Antibody |
| DNA Antibody, Double-Stranded (ENA) | Anti-SSB (La) |
| Anti-SSA (Ro) | Ro Antibody |
| La Antibody | RNP Antibody |
| Anti-Ro (SSA) | Anti-RNP (Ribonucleoprotein) |
| Anti-Sm (Smith) | Sm (Smith) Antibody |
| Anti-La (SSB) | SS-A Antibody |
| SS-B Antibody | Anti-SmRNP |
| Anti-Jo 1 | Scleroderma Antibody |
| Anti-Synthetase Syndrome | Anti-HisRS (Jo-1) |
| Anti-tRNA Synthetase | Sjogrens syndrome-B extractable nuclear Ab |
| Ribonucleoprotein extractable nuclear Ab | Smith extractable nuclear Ab |
SST tube
0.5 mL serum
Minimum Volume:0.25 mL serum
Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Please indicate panel or specific antibody testing to be performed.
Collect specimen in SST tube. Please indicate panel or specific antibody testing to be performed.
Monday - Friday
Analytic Time:1 day
Negative
Useful diagnostic and prognostic information can be gained in ANA positive patients by knowing the specificity of the autoantibodies that are detected. Ideally, collagen vascular disease diagnosis is based on a combination of clinical symptoms and laboratory testing. It is not cost effective to follow up every positive ANA with assays for all 11 possible detectable antibody specificities. MLabs promotes a more cost effective strategy of following positive ANA tests with a quantitative anti-dsDNA assay (order code ADNA) and at initial screening an ENA 5 Antibody Panel (includes SS-A, SS-B, Sm, RNP, and SmRNP) (order code ENA5). This will account for the vast majority of positives. Other ENA antigens such as Scl-70, Centromere B, Jo-1, and Ribosomal P should not be routinely screened for, but ordered as individual tests as the differential diagnosis dictates.
Multiplex Flow Immunoassay
See Extractable Nuclear Antibody Testing.available online at http://www.pathology.med.umich.edu/handbook/Tables/ANA-ENA-08.pdf. ENA5 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, and RNP. ENA7 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, and Scleroderma (Scl-70). ENA11 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, Scleroderma (Scl-70), Centromere B, Chromatin, ds-DNA, and Ribosomal P. The following antibodies may also be ordered individually: Centromere B (CENT B), Jo-1 (JO-1), Ribosomal P (RIBO P), RNP (RNP), Scleroderma (SCL-70), Smith (SMITH), SS-A (SS-A), SS-B (SS-B), and Double Stranded DNA Antibody by RIA (ADNA).
Z32-3 ENA5, Z1002-5 ENA7, Z1003-3 ENA11, 8076-2 JO-1, 8092-9 SCL-70, 16137-2 CENT B, Z1001-7 CHROM, 5130-0 DSDNA, 31592-9 RIBO P
CPT Code86225 DSDNA, 86235 SS-A, SS-B, RNP, SMRNP, SMITH, JO-1, SCL-70, CENT B, CHROM, RIBO P
Inpatient Fee Code20572 ENA5, 20573 ENA7, 36118 ENA11, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
Outpatient Fee Code20572 ENA5, 20573 ENA7, 36118 ENA11, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
MLabs Fee Code20572 ENA5, 20573 ENA7, 36118 ENA11, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
402