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Extractable Nuclear Antibody Panels
Laboratory:
Immunopathology
  Supporting Articles
Soft Order Code:
ENA5/ENA7/ENA10
Synonyms:
SmRNP Antibody U1RNP Antibody
Centromere B Antibody Anti-Centromere
Scl-70 Antibody Anti-Scl 70
Sjogrens syndrome-A extractable nuclear Ab Jo-1 Antibody
Chromatin Antibody Ribosomal Protein Antibody
Anti-SSB (La) Anti-SSA (Ro)
Ro Antibody La Antibody
RNP Antibody Anti-Ro (SSA)
Anti-RNP (Ribonucleoprotein) Anti-Sm (Smith)
Sm (Smith) Antibody Anti-La (SSB)
SS-A Antibody SS-B Antibody
Anti-SmRNP Anti-Jo 1
Scleroderma Antibody Anti-Synthetase Syndrome
Anti-HisRS (Jo-1) Anti-tRNA Synthetase
Sjogrens syndrome-B extractable nuclear Ab Ribonucleoprotein extractable nuclear Ab
Smith extractable nuclear Ab
Container:
SST tube
Normal Volume:
0.5 mL serum
Minimum Volume:
0.25 mL serum
Offsite Collection:
Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Please indicate panel or specific antibody testing to be performed.
Onsite Collection (UMHS Hospitals Only):
Collect specimen in SST tube. Please indicate panel or specific antibody testing to be performed.
Days Set Up:
Monday - Friday
Analytic Time:
1 day
Reference Range:
*Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Negative
Test Usage:
Useful diagnostic and prognostic information can be gained in ANA positive patients by knowing the specificity of the autoantibodies that are detected. Ideally, collagen vascular disease diagnosis is based on a combination of clinical symptoms and laboratory testing. It is not cost effective to follow up every positive ANA with assays for all 10 possible detectable antibody specificities. MLabs promotes a more cost effective strategy an ENA 5 Antibody Panel (includes SS-A, SS-B, Sm, RNP, and SmRNP) (order code ENA5). These antibodies will account for the vast majority of significant positive results. Other ENA antigens such as Scl-70, Centromere B, Jo-1, and Ribosomal P should not be routinely screened for, but ordered as individual tests as the differential diagnosis dictates. The laboratory recommends either anti-dsDNA (order code ADNA) or crithidia DNAIFA (order code DNAIF) to detect and quantify anti-dsDNA.
Test Methodology:
Multiplex Flow Immunoassay
Additional Information:
See Extractable Nuclear Antibody Testing.available online at http://www.pathology.med.umich.edu/handbook/Tables/ANA-ENA-08.pdf. ENA5 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, and RNP. ENA7 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, and Scleroderma (Scl-70). ENA10 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, Scleroderma (Scl-70), Centromere B, Chromatin, and Ribosomal P. The following antibodies may also be ordered individually: Centromere B (CENT B), Jo-1 (JO-1), Ribosomal P (RIBO P), RNP (RNP), Scleroderma (SCL-70), Smith (SMITH), SS-A (SS-A), and SS-B (SS-B).
LOINC:
Z32-3 ENA5, Z1002-5 ENA7, 8076-2 JO-1, 8092-9 SCL-70, 16137-2 CENT B, Z1001-7 CHROM, 5130-0 DSDNA, 31592-9 RIBO P
CPT Code:
86235 EACH ANTIBODY
Inpatient Fee Code:
20572 ENA5, 20573 ENA7, KA002 ENA10, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
Outpatient Fee Code:
20572 ENA5, 20573 ENA7, KA002 ENA10, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
MLabs Fee Code:
20572 ENA5, 20573 ENA7, KA002 ENA10, 32162 RIBO P, 30941 CENT B, 22176 SCL-70, 31528 JO-1
Alternate Specimen:
Red top tube.
Database ID:
402

For Technical Questions or Comments Directly Dealing With The Handbook E-mail: jsica@umich.edu