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BMT Engraftment Analysis, Pre-BMT, Donor
Laboratory:
Molecular Diagnostics
Order Code:
DONBM
Synonyms:
Bone Marrow Transplant Engraftment Evaluation Polymorphism Detection, pre-BMT
Pre-BMT Engraftment Analysis, Donor Pre-BMT Polymorphism Detection
VNTR for pre-BMT Engraftment Analysis Donor BM Engraph. Eval
Chimerism Analysis, Pre-Transplant, Donor
Container:
Lavender top tube
Normal Volume:
14 ml EDTA whole blood; 3 ml EDTA bone marrow
Minimum Volume:
7 ml EDTA whole blood; 1.5 ml EDTA bone marrow
Offsite Collection
Collect blood or bone marrow in lavender top tube. Send intact specimen at room temperature or refrigerated.
Onsite Collection (UMHS Hospitals Only)
Collect blood or bone marrow in lavender top tube. Send intact specimen at room temperature or refrigerated.
Analytic Time:
2 - 7 days
Reference Range:
Interpretive report provided. A combined report for the recipient and donor is issued under the patient (recipient) medical record number; no separate report is issued for the donor.
Test Usage:
To detect genetic polymorphism between the patient (recipient) and the donor prior to the bone marrow transplant, in order to evaluate the engraftment status after the transplant.
Test Limitations:
If the patient has a very low white blood cell count, the DNA yield may be insufficient to complete the analysis. In order to perform this test, both the patient's pre-transplant and the donor's specimen must be available. Specimens submitted for Molecular Diagnostics testing may contain substances that can inhibit PCR gene amplification. If an inhibitor is known to be present, attempts will be made to clear the specimen of inhibitor prior to amplification. Known inhibitors include heparin, medications and some levels of hemoglobin or IgG. The major inhibitor we see in our laboratory is heparin; avoid drawing blood in a heparin tube or through a heparinized port. For molecular diagnostics testing, a lavender top (EDTA) tube is preferred. If specimens containing heparin must be submitted, note "heparin" on the requisition to alert the lab to 'clean' the specimen before analysis thus reducing turnaround time, improving retention of DNA in the specimen and increasing our ability to return a conclusive result.
Test Methodology:
Multiplex Polymerase Chain Reaction (PCR)
Additional Information:
By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.
Database ID:
755

For Technical Questions or Comments Directly Dealing With The Handbook E-mail: jsica@umich.edu