CLINITESTŪ Reagent Tablets are based on
the classic Benedict’s copper reduction reaction, combining reactive
ingredients with an integral heat generating system.
Copper sulfate in CLINITEST reacts with reducing substances in urine,
converting cupric sulfate to cuprous oxide. The resultant color, which
varies with the amount of reducing substance present, ranges from blue
through green to orange. Heat required is furnished by the reaction of
sodium hydroxide with water and with citric acid. Sodium carbonate and
citric acid help to dissolve the tablet.
The Chemstrip glucose pad is only specific for the detection of increased
levels of glucose and will not detect increased levels of other reducing
substances (galactose, fructose, lactose, and pentose). A copper reduction
test like the CLINITEST detects some hereditary disorders, which may be
present in pediatric patients.
12 ml fresh urine with no preservatives
Refrigerate urine if testing cannot be done immediately
A number of substances found in urine, such as salicylates and penicillin,
react positively with CLINITEST Reagent Tablets but are not present in most
cases in sufficient quantity to interfere with the test. Ascorbic acid,
nalidixic acid, cephalosporins and probenecid in large quantities may cause
false positive results.
Negative (Blue color)
Urine containing more than 2% sugar using the 5-drop method may cause a very
rapid color change during the boiling and 15-second waiting period. OBSERVE
the solution closely during this time to detect “pass-through” color
changes. Should these occur, the color will pass rapidly through bright
orange to a dark brown or greenish brown. In this case, record the
result as “greater than 2%” sugar.
Low specific gravity urines containing glucose may give slightly
High protein concentrations extend the boiling time, increase foaming and
may make visual comparison difficult.
The presence of X-ray Contrast Media (Hypaque meglumine) in urine affects
CLINITEST Reagent Tablets producing reduced glucose results
Pathology performs quality control check using Alta Positive and Negative
Controls whenever a container is first opened to confirm the performance of the
reagent tablets. Do not report any patient results when quality control results
do not match Quality Control criteria. Repeat test and discard tablets that do
not meet quality control standards.
1. With dropper in upright position, place 5 drops of urine in a test tube.
2. Rinse dropper with water and add 10 drops of water to test tube.
3. Drop one Clinitest tablet into test tube. Watch while complete boiling
reaction takes place. Do not shake test tube during boiling, or for the
following 15 seconds after boiling has stopped.
4. At the end of this 15-second waiting period, shake test tube gently to mix
contents. Compare color of liquid to Color Chart provided on the package
insert. Ignore sediment that may form in the bottom of the test tube. Ignore
changes after the 15-second waiting period.
5. Record the percent (%) result which appears on the color block that most
closely matches the color of the liquid.
Record the percent (%) results which appears on the color chart that most
closely matches the color of the liquid after 15-second waiting period.
NOTE: CLINITEST is not specific for glucose and will react with sufficient
quantities of any reducing substance in the urine. Failure to observe the
reaction at all times can lead to erroneously low results if the “pass-through”
phenomenon is missed.
CLINITESTŪ Reagent Tablets (100 tablets Product No. 2126)
1. Contains 1 part copper sulfate, 12 parts sodium hydroxide, 4 parts sodium
carbonate, 15 parts citric acid, filler and binder.
2. Prolonged stability in the UNOPENED container, either in the glass bottle
or foil-wrapped, if stored at room temperature (59-86oF or 15-30oC).
3. Once the bottle is opened, stability is STRONGLY dependent on protection
from moisture. Tablets should be used on a regular basis and not stored for
extended periods of time after the bottle is opened.
4. PROTECT CLINITEST TABLETS FROM LIGHT, HEAT AND MOISTURE. Moisture causes
CLINITEST tablets to turn a deeper shade of blue. If tablets darken, or if test
results seem questionable or inconsistent
with expected findings: 1) Confirm that product is within expiration date shown
on label or foil; 2) Check performance with a positive control. If proper result
is not obtained, discard and retest with fresh CLINITEST Reagent Tablets.
NOTE: Contains poison; causes severe burns
Warnings: Contains sodium hydroxide (caustic soda). Avoid contact with skin,
eyes, mucous membranes and clothing. CLINITEST tablets are highly sensitive to
moisture from air or water. Excessive moisture may cause a chemical reaction
and a bottle explosion may occur.
Product insert, CLINITESTŪ Reagent Tablets, Bayer Corporation,
Elkhart, IN, 9/95.