Laboratory:
Chemical Pathology
Referral Laboratory:
MiChart Code:
Syphilis Antibody, Treponema pallidum Particle Agglutination, Serum
Soft Order Code:
TPPA
Synonyms:
Container:
SST Preferred Red Top acceptable EDTA acceptable- must be received in lab within 48 hours
Normal Volume:
1.5 mL serum 1.5 mL plasma
Minimum Volume:
1.0 mL serum 1.0 mL plasma
Offsite Collection:
Collect specimen in SST tube or EDTA, if necesary. Centrifuge, aliquot serum into plastic vial and refrigerate. Store at 2-8C up to 5 days. Freeze serum for longer storage.
Onsite Collection (Michigan Medicine Hospitals Only):
Collect specimen in SST tube.
Days Set Up:
Tuesday, Friday
Analytic Time:
8 hours
Reference Range:
*Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Non-reactive
Test Usage:
Aids in the diagnosis of syphilis by confirming presence of Treponema pallidum antibodies.
Test Limitations:
TPPA may be reactive in persons from areas where yaws and pinta is or was endemic. Samples from patients with HIV, Leprosy, Toxoplasmosis, H. Pylori, and drug addiction may react with test particles causing false-positive or inconclusive results.
Test Methodology:
Particle agglutination
Additional Information:
The identification of Treponema pallidum antibodies aids in the diagnosis of syphilis caused by the microorganisms belonging to the genus Treponema and provides epidemiological information on syphilis. A reactive treponemal test indicates past or present infection and usually remains positive for life. A non-reactive result indicates no past or present infection, but may occur during incubation-stage syphilis.
LOINC:
24312-1
CPT Code:
86780
Fee Code:
KA050
Rejection Criteria:
Samples frozen and thawed more than once.
Storage Temperature:
2-8C
Test ID:
5764