Laboratory:
Sendout
Referral Laboratory:
Mayo LPMGF (60591)
MiChart Code:
Lymphocyte Proliferation Mitogens
Soft Order Code:
LBMIT
Synonyms:
Blastogenesis, Mitogens Lymphocyte Clastogenesis Mitogens
Lymphocyte Transformation Mitogen Studies
Lymphocyte Mitogen Stimulation Leukocyte Function Assay
Lymphocyte Blastogenesis, Mitogens Mitogen Cell Cycle Analysis
Lymphocyte Mitogen Proliferation Analysis
Container:
Green top tubes (sodium heparin only) No lithium heparin
Normal Volume:
18 years: 10 mL
Minimum Volume:
1 mL
Special Handling:
Draw Monday- Thursday only, and specimen must be received in Sendouts by 6pm Thursday at the latest.
Offsite Collection:
Please notify MLabs Client Services Center prior to sending specimen. Specimens are accepted Monday through Thursday only and must be received by performing laboratory within 24 hours of collection (specimen must be received by the University Hospital main lab sendout area by 6:00 pm Thursday). Collect blood in two green top sodium heparin tubes; do not use tubes containing lithium heparin. Send whole blood at room temperature; refrigerated or frozen specimens are unacceptable.
Onsite Collection (Michigan Medicine Hospitals Only):
Collect specimen in green top (sodium heparin only) tubes. Send intact specimen at room temperature on the day of collection. Specimens are accepted Monday through Thursday only and must be received by performing laboratory within 24 hours of collection (specimen must be received by Specimen Processing in the University Hospital main lab by 6:00 pm Thursday). Do not refrigerate or freeze.
Days Set Up:
Monday - Friday
Analytic Time:
8 - 11 days
Reference Range:
*Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Viability of Lymphocytes at Day 0: >=75%; Max Prolif of PHA as %CD45: >=49.9%; Max Prolif of PHA as %CD3: >=58.5%; Max Prolif of PWM as %CD45: >=4.5%; Max Prolif of PWM as %CD3: >=3.5%; Max Prolif of PWM as %CD19: >=3.9%.
Test Usage:
Diminished responses to lectin mitogens are consistent with a primary or secondary immunodeficiency disease. Abnormal results are not specific for a particular disease, and the magnitude of the abnormality is not necessarily related to the degree of immunodeficiency.
Test Methodology:
Flow Cytometry
Additional Information:
Test sent to Mayo Medical Laboratories.
LOINC:
S-1060
CPT Code:
86353
Fee Code:
20099
Alternate Specimen:
ACD tube is acceptable (Green top preferred, sodium heparin only).
Rejection Criteria:
Specimen collected in lithium heparin green top tubes
Storage Temperature:
Strict ambient
Test ID:
607