Laboratory:
Sendout
Referral Laboratory:
Mayo CIFS (8052)
MiChart Code:
Cutaneous Immunofluorescence Antibodies, Serum (Sendout)
Soft Order Code:
CIFS
Synonyms:
Skin Antibodies (Pemphigus and Pemphigoid), Serum Paraneoplastic pemphigus
Pemphigus vulgaris Epidermal Antibodies
Bullous lupus erythematosus Linear IgA disease
Cicatricial pemphigoid Herpes gestationis
Epidermolysis bullosa acquisita Bullous pemphigoid
Pemphigus
Container:
Red top or SST tube
Normal Volume:
2 mL serum
Minimum Volume:
0.5 mL serum
Offsite Collection:
Collect blood in a red top or SST tube. Centrifuge, aliquot serum into a plastic vial, and refrigerate. Include patient history on requisition. Indicate possible diagnosis on requisition: Pemphigus, Bullous Pemphigoid, Cicatricial Pemphigoid, Epidermolysis Bullosa Acquisita, Herpes gestationis, Linear IgA Disease, or Other.
Onsite Collection (Michigan Medicine Hospitals Only):
Include patient history on requisition. Check off possible diagnosis on requisition: Pemphigus, Bullous Pemphigoid, Cicatricial Pemphigoid, Epidermolysis Bullosa Acquisita, Herpes gestationis, Linear IgA Disease, Other.
Days Set Up:
Monday - Friday
Analytic Time:
5 - 7 days
Reference Range:
*Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Negative in normal individuals. Report includes presence and titer of circulating antibodies. If serum contains anti-basement membrane zone (BMZ) antibodies on split-skin substrate, patterns will be reported as: 1) epidermal pattern, consistent with pemphigoid or 2) dermal pattern, consistent with epidermolysis bullosa acquisita.
Test Usage:
Confirming a diagnosis of pemphigoid, pemphigus, EBA, or bullous LE. Monitoring therapeutic response in patients with pemphigus.
Test Limitations:
Results should be interpreted in conjunction with clinical information, histologic pattern, and results of direct IF study. In particular, the finding of low titer (1:20 or 1:40) IgG anti-cell surface (CS) antibodies should not be used alone (i.e., without histologic or direct IF support) to confirm a diagnosis of pemphigus.
Test Methodology:
Detection of IgG anti-intercellular substance (ICS) and anti-basement membrane zone (BMZ) antibodies by indirect immunofluorescence technique using Rhesus monkey esophagus substrate and human NaCl split-skin substrate.
Additional Information:
Test sent to Mayo Medical Laboratories.
CPT Code:
88346, 88350
Fee Code:
AA435, AA436
Template Code:
SKAB
Test ID:
848