Proteomics Resource Facility

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Instrumentation

The PRF is equipped with two Thermo Scientific mass spectrometers devoted exclusively for proteomic analysis.  These include:

  1. Orbitrap Fusion Tribrid with ETD
  2. Q Exactive HF

    Note: Both mass spectrometers are equipped with dedicated RSLC-nano (Dionex) UHPLCs and nanospray sources. 

                                     

 

  • Orbitrap Tribrid Fusion with ETD:  (Acquired with funding from the Protein Folding DIsorder Initiative, a part of University of Michigan's Strategic Research Initiative).  
    • The Thermo Scientific™ Orbitrap Fusion™ Tribrid™ mass spectrometer combines the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture that delivers unprecedented depth of analysis. It enables life scientists analyzing even the most challenging low-abundance, high-complexity, or difficult samples to identify more compounds more quickly, quantify more accurately, and elucidate structures more thoroughly.  The instrument can perform parallel CID, HCD, ETD, EThcD and ETciD fragmentation analysis to provide the most in depth information about the complex samples. 

                    

                Orbitrap-Fusion.jpg                                               Schematic of the Orbitrap Fusion Tribrid MS (taken from http://planetorbitrap.com)

     

  • Q Exactive HF

 

 

                 QE HF.jpg                   Q Exactive HF_Schematic.jpg