Pathology Flow Cytometry Core Laboratory

The FACSCelesta flow cytometer (7522 MSRB1) has three lasers and can analyze fourteen parameters (twelve colors plus light scatter). A BD High-Throughput Sampler (HTS) unit capable of taking samples from 96-well plates is coupled to the cytometer.  Users are allowed to switch between 96-well plates and 12x75 mm tubes as their experimental needs warrant.  A brief training video has been produced demonstrating how to do this.  The software used on the instrument is Becton-Dickinson's "FACSDiva," version 8. 

 

An ACEA Biosciences "NovoCyte" instrument was acquired in the fall of 2023 and is located in 4661 BSRB.  The NovoCyte is capable of analyzing twenty-five parameters (24 colors plus light scatter).  A list of available detectors will be linked to this page in the future.  Similar to the LSRII, there are three lasers:  a 405 nm "violet", a 488 nm "blue", and a 640 nm "red."  Additionally there is a 561nm "yellow-green" laser.   A handy feature of the NovoCyte is that can accept either 12x75 mm tubes or 96-well plates.